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ZP1 and ZFP8 physically interact with CLF and SWN in Arabidopsis . ZFP8 physically interacts with CLF ( A ) and SWN ( B ). Arabidopsis protoplasts were transfected with CLF-FLAG (A) or SWN-FLAG (B) and ZFP8-HA or ZFP8(-E)-HA, or an empty vector (Control), and subjected to IP with anti-HA magnetic beads, followed by immunoblotting with anti-HA and anti-FLAG antibodies. ( C ) ZP1 physically interacts with CLF and SWN in Arabidopsis protoplasts. Arabidopsis protoplasts were transfected with CLF-FLAG or SWN-FLAG, or an empty vector (Control), and ZP1-HA or ZP1(-E)-HA were subjected to IP with anti-FLAG magnetic beads, followed by immunoblotting with anti-HA and anti-FLAG antibodies. ( D ) ZFP8 associates with FIE in transgenic Arabidopsis plants. Two-week-old transgenic plants expressing pZFP8::ZFP8-GFP and pFIE::FIE-HA were subjected to IP with anti-GFP magnetic beads, followed by immunoblotting for GFP and HA.

Journal: Nucleic Acids Research

Article Title: ZINC FINGER PROTEIN 1 and 8 interact with polycomb repressive complex 2 to repress class B and C floral organ identity genes

doi: 10.1093/nar/gkag045

Figure Lengend Snippet: ZP1 and ZFP8 physically interact with CLF and SWN in Arabidopsis . ZFP8 physically interacts with CLF ( A ) and SWN ( B ). Arabidopsis protoplasts were transfected with CLF-FLAG (A) or SWN-FLAG (B) and ZFP8-HA or ZFP8(-E)-HA, or an empty vector (Control), and subjected to IP with anti-HA magnetic beads, followed by immunoblotting with anti-HA and anti-FLAG antibodies. ( C ) ZP1 physically interacts with CLF and SWN in Arabidopsis protoplasts. Arabidopsis protoplasts were transfected with CLF-FLAG or SWN-FLAG, or an empty vector (Control), and ZP1-HA or ZP1(-E)-HA were subjected to IP with anti-FLAG magnetic beads, followed by immunoblotting with anti-HA and anti-FLAG antibodies. ( D ) ZFP8 associates with FIE in transgenic Arabidopsis plants. Two-week-old transgenic plants expressing pZFP8::ZFP8-GFP and pFIE::FIE-HA were subjected to IP with anti-GFP magnetic beads, followed by immunoblotting for GFP and HA.

Article Snippet: FIE-HA and ZFP8-GFP protein were detected by western blotting using anti-HA (11583816001; Sigma), anti-GFP (632381; Takara), and HRP-linked anti-mouse IgG (7076; Cell Signalling) antibodies, respectively.

Techniques: Transfection, Plasmid Preparation, Control, Magnetic Beads, Western Blot, Transgenic Assay, Expressing